Evaluation of potential biological activities of metabolites from endophytic fungi residing in leaves of Azadirhacta indica

The objective of the present study is to evaluate anti bacterial activity against Bacillus sp, Pseudomonas aeruginosa and Staphylococcus aureus anti tumour activity against Hep 2 cell line with crude metabolites isolated from endophytic fungi residing in leaves of Azadirhacta indica. Endophytic fungi isolated from sterile leaflets of Azadirhacta indica and the isolated respective fungi were grown in liquid media, the metabolites were obtained from culture free supernatant of the media after the extraction with ethyl acetate, the concentrated extract used as crude metabolite, the metabolites thus obtained with different concentration evaluated against bacterial strains adopting agar diffusion assay. Minimum inhibitory concentration against the tested bacterial strains was studied adopting broth dilution method. Anti tumour activity was studied against HEP 2 cell line adopting MTT assay..Cytotoxic effect of metabolites on vero cell line adopting MTT assay and human peripheral blood RBC with microscopic examination was also studied. A total of 25 isolates belong to Alternaria, Cladosporium, Paecilomyces, Rhizopus, Trichoderma sp were isolated. Among the different fungal extracts, ethyl acetate extract of Cladosporium sp showed anti bacterial and anti tumour activity. All the tested bacteria were susceptible to ethyl acetate extract and maximum activity was recorded in Pseudomonas aeruginosa.Anti tumour activity against HEP 2 cell line reveals 100 μg concentration inhibited maximum viability of HEP 2 cell line. Distinct cytotoxic effect on vero cell line and RBC was not recorded. The study suggests the possible utilization of endophytic fungal metabolites of Cladosporium sp against bacterial strains and cancer.